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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Respiratory Pathogen Panel 
  Synonym: Virus Respiratory PCR, FilmArray Respiratory Panel 
  Method: Qualitative nucleic acid amplified multiplex PCR assay 
  Availability: Varies according to test volume; refer to laboratory for testing interval. Specimens received by 0400 on testing days will have same day results. 
  Specimen: Nasopharyngeal wash, nasopharyngeal swab, or BAL 
  Collection Device: Universal viral transport media (1 - 2 mL), Starswab viral transport tube, or sterile tube for BAL. 
  Volume: Collect 2 - 3 mL nasopharyngeal wash (using normal saline) or 2 - 3 mL BAL 
  Storage/Transport: Store refrigerated and transport within 72 hours 
  Unacceptable: Slides, smears; unacceptable specimens (sputum; tracheal aspirate; lung tissue biopsy; and pleural fluid); samples collected in non-viral transport media or using a bacterial culture swab; dry swabs; wood shaft or calcium alginate swabs; samples collected in EIA or ProbeTec transport media 
  Specimen Stability: Refrigerated, 72 hours 
  Reference Interval: Negative for Influenza Type A virus, Influenza A subtype H1 virus (seasonal), Influenza A subtype H1 virus (pandemic 2009), Influenza A subtype H3 virus (seasonal), Influenza B virus, Respiratory Syncytial Virus (RSV), Parainfluenza viruses (1, 2, 3 and 4), Human Rhinovirus/Enterovirus, Human Metapneumovirus, Adenovirus, Coronavirus (HKU1, NL63, 229E, and OC43), Bordetella pertussis, Chlamydophila pneumoniae, and Mycoplasma pneumoniae 
  Reportable Disease: A positive Adenovirus, Influenza A virus, Influenza B virus, RSV, or Bordetella pertussis result is reportable 
  Comments: This test is now being recommended for the management of severely ill or hospitalized patients. Subtyping influenza may be helpful in predicting antiviral susceptibility. 
  Revised: 10/3/2013 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.