Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Rubella Virus Antibody, IgM 
  Method: Chemiluminescent Immunoassay 
  Availability: Sunday-Saturday; results within 24 hours 
  Specimen: Blood 
  Collection Device: 6.0 mL Gold (SST) tube 
  Volume: 1.0 mL serum (Min: 0.5 mL) 
  Storage/Transport: Refrigerate; allow specimen to clot and separate from cells and transfer to clean tube 
  Unacceptable: Contaminated, heat-inactivated, or hemolyzed samples. 
  Specimen Stability: Refrigerated, 2 weeks; frozen, 1 year; avoid multiple freeze/thaw cycles 
  Reference Interval: < 0.89 IV: Negative - No significant level of detectable rubella IgM antibody.
0.90 - 1.09 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
> 1.10 IV: Positive - IgM antibody to rubella detected, which may indicate a current or recent infection or immunization.
  Reportable Disease:  
  Comments: Acute and convalescent samples must be labeled as such. Parallel testing is preferred and convalescent samples must be received within 30 days from receipt of acute samples. Please mark samples plainly as "acute" or "convalescent." Testing immediately post-exposure is of no value without a later convalescent specimen. While the presence of IgM antibodies suggests current or recent infection, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection or immunization. 
  Revised: 7/7/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.