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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Mumps Virus Antibody, IgG 
  Synonym: Mumps Virus Titer 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Weekly on Wednesdays (if specimen received by 0800); results on same day 
  Specimen: Blood 
  Collection Device: One 6.0 mL Clot (Red) or one 6.0 mL SST (Gold) tube. 
  Volume: 1 - 4 mL blood (0.5 - 2.0 mL serum) 
  Storage/Transport: Separate serum ASAP; store and transport refrigerated, and if transport > 48 hours, freeze 
  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles 
  Specimen Stability: After separation from cells: ambient, < 8 hours; refrigerated, 48 hours; frozen, 1 year 
  Reference Interval: Negative: no detectable mumps virus IgG antibody

Equivocal: borderline levels of IgG antibody to mumps virus. Repeat testing in 10-14 days recommended.

Positive: mumps virus IgG antibody detected. Indicative of current or past infection. Positive IgG antibody levels in the absence of current clinical symptoms may also indicate immunity. 
  Reportable Disease: A four-fold rise in titer between the acute and convalescent sera is reportable (see comment) 
  Comments: Confirmation of mumps by detection of IgG antibody levels requires testing of paired sera. Acute and convalescent sera with index values of < 0.90 and > 1.10, respectively have been shown to represent a ≥ 4-fold increase in titer. In cases where an acute serum index value is > 0.90, a 1.6 fold increase in index ratio (convalescent serum IV/ acute serum IV) is indicative of a significant antibody increase. Index ratios between > 1.4 and < 1.6 are considered equivocal and submission of a new convalescent serum after two weeks is recommended. For additional interpretive information, contact the laboratory. When paired testing is considered, convalescent specimens must be received within 30 days from receipt of acute specimens. 
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.