||Mumps Virus Antibody, IgG
||Mumps Virus Titer
||Enzyme-Linked Immunosorbent Assay
||Weekly on Wednesdays (if specimen received by 0800); results on same day
| Collection Device:
||One 6.0 mL Clot (Red) or one 6.0 mL SST (Gold) tube.
||1 - 4 mL blood (0.5 - 2.0 mL serum)
||Separate serum ASAP; store and transport refrigerated, and if transport > 48 hours, freeze
||Severely lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles
| Specimen Stability:
||After separation from cells: ambient, < 8 hours; refrigerated, 48 hours; frozen, 1 year
| Reference Interval:
||Negative: no detectable mumps virus IgG antibody
Equivocal: borderline levels of IgG antibody to mumps virus. Repeat testing in 10-14 days recommended.
Positive: mumps virus IgG antibody detected. Indicative of current or past infection. Positive IgG antibody levels in the absence of current clinical symptoms may also indicate immunity.
| Reportable Disease:
||A four-fold rise in titer between the acute and convalescent sera is reportable (see comment)
||Confirmation of mumps by detection of IgG antibody levels requires testing of paired sera. Acute and convalescent sera with index values of < 0.90 and > 1.10, respectively have been shown to represent a ≥ 4-fold increase in titer. In cases where an acute serum index value is > 0.90, a 1.6 fold increase in index ratio (convalescent serum IV/ acute serum IV) is indicative of a significant antibody increase. Index ratios between > 1.4 and < 1.6 are considered equivocal and submission of a new convalescent serum after two weeks is recommended. For additional interpretive information, contact the laboratory.
When paired testing is considered, convalescent specimens must be received within 30 days from receipt of acute specimens.