Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Norovirus RNA Direct Detection 
  Synonym: Norwalk virus PCR, Human Calicivirus PCR 
  Method: Qualitative Reverse Transcription Real-Time DNA Amplification 
  Availability: Twice weekly; results reported within 3 days 
  Specimen: Stool 
  Collection Device: Sterile Container 
  Volume: Minimum 1 mL stool 
  Storage/Transport: Refrigerate after collection (if 3 days or less) or freeze 
  Unacceptable: Stool stored at room temperature greater than 8 hours and stool contaminated with urine 
  Specimen Stability: Ambient, 8 hours; refrigerated, 3 days; frozen, 1 month 
  Reference Interval: No norovirus detected. Results for genotype I and II reported if positive. 
  Reportable Disease: Positive result is reportable 
  Comments: Although 4 genotypes of noroviruses are known, only genotypes I and II are detected by this assay. A negative result does not rule out the presence of PCR inhibitors (heme) in the patient specimen or norovirus concentrations below the level of detection of the assay. 
  Revised: 7/1/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.