Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

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  Test: Norovirus RNA Direct Detection
Order in NUlirt or complete NPHL Test Order Form  
  Synonym: Norwalk virus PCR, Human Calicivirus PCR  
  Method: Real-time DNA Amplification  
  Availability: Test performed Monday – Friday; Turn-around-time 24-48hr  
  Specimen: Stool  Specimen  
  Collection Device: Sterile Container
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: Minimum 1 mL  
  Storage/Transport: Prior to and during shipment: Refrigerate 2-8°C for up to 72h after collection. Ship as soon as possible.
Shipping instructions, including specimen-handling requirements during transport: All screw-top tubes must be sealed with Parafilm™ or tape.  
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimen should be shipped on frozen gel-packs. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.    

See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.  
  Unacceptable: Stool stored at room temperature >8 hours and stool contaminated with urine  
  Specimen Stability: Ambient, 8 hours; refrigerated, 3 days; frozen, 1 month   
  Reference Interval: No norovirus detected. Results for genotype I and II reported if positive  
  Reportable Disease: Presumptive positive results are reported to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.  
  Comments: Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.
The Cepheid Xpert Norovirus Assay, performed on the GeneXpert® Instrument Systems, is a qualitative in vitro diagnostic test for the identification and differentiation of norovirus genogroup I and genogroup II RNA from raw or unpreserved uniformed stool specimens collected from individuals with symptoms of acute gastroenteritis. The test utilizes automated real-time reverse transcriptase polymerase chain reaction (RT-PCR) to detect norovirus.  
  Revised: 3/21/2021 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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