Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Hepatitis B Core Antibody, Total IgG, IgM  
  Synonym: Antibody to Hepatitis B Core Antigen; Anti-HBc; Core Antibody 
  Method: Chemiluminescent Immunoassay  
  Availability: Daily; results in 24 hours  
  Specimen: Blood  
  Collection Device: 6.0 mL SST (Gold) tube or 5.0 mL EDTA (Lavender) tube  
  Volume: 0.5 mL serum or plasma (Min: 0.3 mL)  
  Storage/Transport: Refrigerate; if sample is not transported to lab the same day as collected aliquot the serum or plasma into a clean tube and refrigerate, labeling tube with "serum" or "plasma."  
  Unacceptable: Heparinized plasma  
  Specimen Stability: Ambient, < 12 hours; refrigerated, 72 hours; frozen, 1 year
Avoid multiple freeze/thaw cycles  
  Reference Interval: Nonreactive  
  Reportable Disease:  
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.