Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Hepatitis B Surface Antibody 
  Synonym: Antibody to Hepatitis B Surface Antigen; Anti-HBs; Australian Antigen Antibody 
  Method: Chemiluminescent Immunoassay 
  Availability: Daily; results in 24 hours 
  Specimen: Blood 
  Collection Device: 6.0 mL SST (Gold) tube or 5.0 mL EDTA (Lavender) tube 
  Volume: 1.0 mL serum or plasma (Min: 0.5 mL) 
  Storage/Transport: Refrigerate; if sample is not transported to lab the same day as collected aliquot the serum or plasma into a clean tube and refrigerate, labeling tube with "serum" or "plasma." 
  Unacceptable: Heparinized plasma 
  Specimen Stability: Ambient, < 12 hours; refrigerated, 72 hours; frozen, 1 year
Avoid multiple freeze/thaw cycles 
  Reference Interval: Nonreactive 
  Reportable Disease:  
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.