Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: West Nile Antibody IgG & IgM, CSF 
  Synonym: West Nile Virus Antibody 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Varies by season. In-season testing is performed on Tuesday with results for specimens received by 0800 reported the next day by 1700. For off-season testing results will be available within 14 days of receipt of specimen in lab. 
  Specimen: CSF 
  Collection Device: Sterile, capped tube 
  Volume: 1.0 mL 
  Storage/Transport: Refrigerate; if transport to the lab is not the same day as collection, freeze 
  Specimen Stability: Ambient, not stable; refrigerated, < 24 hours; frozen, 1 month 
  Reference Interval: IgG: Negative
IgM: Negative 
  Reportable Disease:  
  Comments: The Nebraska State Epidemiologist does not recommend screening for only West Nile IgG antibody. All requests for only IgG wil be sent to a reference laboratory and will be charged to the submitter. 
  Revised: 2/3/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.