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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Borrelia burgdorferi Antibodies, Total 
  Synonym: Lyme Antibodies 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Sunday and Tuesday - Saturday; results in 1-3 days 
  Specimen: Blood 
  Collection Device: 6.0 mL Clot (Red) or SST (Gold) tube 
  Volume: 2.0 mL serum (Min: 0.1 mL)  
  Storage/Transport: Refrigerate; separate from cells ASAP 
  Unacceptable: CSF, severely lipemic, heat-inactivated, contaminated, or hemolyzed samples.  
  Specimen Stability: After separation from cells, ambient, not stable; refrigerated, 2 weeks; frozen, 1 year. Avoid multiple freeze/thaw cycles. 
  Reference Interval: < 1.00 LIV: Negative - Antibody to Borrelia burgdorferi not detected.
1.00-1.20 LIV: Equivocal - Repeat testing in 10-14 days may be helpful.
> 1.20 LIV: Positive - Probable presence of antibody to Borrelia burgdorferi detected. 
  Reportable Disease:  
  Comments: Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with Western blot. Both IgM and IgG Western blots should be performed on samples less than four weeks after appearance of erythema migrans. Only IgG Western blot should be performed on samples greater than four weeks after the disease onset. IgM Western blot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate Western blot testing within 10 days. Once this test is performed, if a) Negative - no further testing is done, b) Positive or equivocal - Western blot testing will be performed on the original sample upon receiving a request. Sample will be held for 30 days only. 
  Revised: 2/2/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.