Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Corynebacterium diphtheriae Culture 
  Synonym: Diphtheriae Culture 
  Method: Standard reference procedure for C. diphtheriae culture and identification 
  Availability: Daily; results in 72 hours 
  Specimen: Swab of nasopharynx plus swab of throat at site of membrane, inflammation, or wound 
  Collection Device: Culturette swab with Stuart's or Amies media 
  Storage/Transport: Ambient; must be received by lab within 24 hours of collection 
  Unacceptable: Specimens older than 24 hours; refrigerated or frozen specimens 
  Specimen Stability: Stable at ambient 
  Reference Interval: Negative 
  Reportable Disease:  
  Comments: If collected from wound, clean wound first 
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.