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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Influenza Type A and Influenza Type B Antigen Detection 
  Synonym: Direct Influenza Antigen Test 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Daily, on all shifts. Routine results within 4 hours and stat results within 1 hour of specimen receipt in the laboratory. 
  Specimen: Nasopharyngeal wash or nasopharyngeal swab 
  Collection Device: Universal viral transport media (1 - 2 mL) or Starswab viral transport tube 
  Volume: Place 2 - 3 mL nasopharyngeal wash into normal saline 
  Storage/Transport: Store refrigerated and transport within 72 hours 
  Unacceptable: Slides, smears; unacceptable specimens (bronchial washing or BAL; sputum; tracheal aspirate; lung tissue biopsy; and pleural fluid); samples collected in non-viral transport media or using a bacterial culture swab; dry swabs; wood shaft or calcium alginate swabs; samples collected in EIA or ProbeTec transport media. 
  Specimen Stability: Refrigerated, 72 hours 
  Reference Interval: Negative for Influenza Type A and Influenza Type B viruses

A negative screen does not rule out the presence of influenza virus 
  Reportable Disease: A positive result is reportable 
  Comments: A rapid EIA for influenza A and B detects both influenza A virus (including the 2009 A H1N1v) and influenza B virus, but does not subtype. 
  Revised: 7/1/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.