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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Fungus Culture, General 
  Synonym: Mycology Culture, Mold Identification 
  Method: Standard reference procedures for fungal culture; DNA probes available on isolate for Histoplasma, Coccidioides, and Blastomyces 
  Availability: Daily; preliminary report at 2 weeks; final report at 6 weeks for tissues, biopsies, and bone marrow and 4 weeks for all other sources 
  Specimen: Tissue, pus, purulent fluid, respiratory specimens, body fluid, skin biopsy 
  Collection Device: Sterile container or culturette swab 
  Volume: 5.0 mL fluid minimum 
  Storage/Transport: Transport and store specimens from sterile body sites (fluids, tissues, etc.) at ambient temperature and refrigerate specimens from nonsterile sites (respiratory, GI tract, etc.) 
  Unacceptable: Non-sterile or leaking container, insufficient volume 
  Specimen Stability:  
  Reference Interval: No fungi isolated at 6 weeks 
  Reportable Disease:  
  Comments: Indicate suspected organism(s) on the test request form. Additional patient history may be helpful; include the patient's occupation, history of travel or residence abroad, and any animal contact. Media is available for direct inoculation. If CSF cannot be transported within 8 hours, hold at 35 °C until the time of transport. For blood specimens, refer to Fungus Culture, Blood. Notification of positive results for mold or positive CSF cultures is provided. For skin, hair, and nail specimens see Fungus Culture Dermatophyte. 
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.