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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: HIV RNA Quantitation, Ultrasensitive 
  Synonym: HIV Viral Load Ultrasensitive 
  Method: Quantitative Nucleic Acid Amplification 
  Availability: Test performed at least once/week; results within 1 week 
  Specimen: Blood 
  Collection Device: 7.0 mL EDTA (Lavender) tube 
  Volume: 3.0 mL plasma minimum 
  Storage/Transport: Frozen; centrifuge and aliquot plasma to a clean tube within 6 hours of collection, labeling with patient name, date of birth and "EDTA Plasma." 
  Specimen Stability: Specimen stable frozen 
  Reference Interval: HIV-1 RNA not detected, < 50 copies/mL 
  Reportable Disease:  
  Comments: This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels. The analytic measurement range of this assay is 1.7 - 7.0 log copies (50 - 10,000,000 copies/mL). The clinical significance of changes in HIV-1 RNA measurement has not been fully established; however, a threefold (0.5 log) change in copies/mL may be significant. False positive results may occur. Care should be taken when interpreting results, especially for seronegative patients. This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues, and Cellular Tissue-Based Products (HCT/P). 
  Revised: 7/11/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.