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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at nphl@unmc.edu or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

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  Test: HIV 1,2 Antibody Confirmation EIA 
  Synonym: HIV confirmation, HIV Monospot 
  Method: Rapid enzyme immunoassay 
  Availability: Monday - Friday, within 72 hours of positive HIV Antigen/Antibody screen 
  Specimen: Blood 
  Collection Device: 6 ml SST (Gold) tube, 6 ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube 
  Volume: Minimum 0.5 ml serum or plasma 
  Storage/Transport: Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube. 
  Unacceptable: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours 
  Specimen Stability: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours 
  Reference Interval: Nonreactive 
  Reportable Disease: Reactive is reportable 
  Comments: Indeterminate results are reflex tested by the HIV RNA assay.

All HIV 2 reactive results will be confirmed by the CDC. 
  Revised: 2/28/2017 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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