Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Vibrio Culture  
  Synonym: Cholera culture 
  Method: Standard reference procedures for Vibrio culture and identification  
  Availability: Daily; final negative report at 2 days, positives called as soon as detected  
  Specimen: Preserve stool in maintenance media (Cary-Blair or Enteric Plus) after collection  
  Collection Device: Sterile stool container with maintenance media

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: 10.0 mL stool in maintenance media  
  Storage/Transport: Prior to and during shipment: Ambient 15-25°C

Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address:  
  Unacceptable: Nonsterile or leaking container, multiple specimens in 24 hour period, dry specimens, diapers, or delayed transport to lab in inappropriate preservative  
  Specimen Stability: Ambient 15-25°C for 72 h  
  Reference Interval: Culture negative for Vibrio spp
  Reportable Disease: Positive result should be immediately reported to local and state health department and isolates should be forwarded to the NPHL, refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send documented approval to or call (402) 559-9444.

Indicate suspected organisms and available patient history, especially any recent travels, on the test request form. Maintenance media is available from Client Services.  
  Revised: 7/11/2019 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.