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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Vibrio Culture 
  Synonym: Cholera culture, Culture, Vibrio 
  Method: Standard reference procedure for Vibrio culture and identification 
  Availability: Daily; results within 3 days 
  Specimen: Stool 
  Collection Device: Stool container or Cary-Blair medium preservative 
  Volume: 1.0 - 5.0 mL fresh stool or 10 mL in Cary-Blair medium 
  Storage/Transport: Transport stool unpreserved at ambient temperature. If transport delayed greater than 2 hours, preserve in enteric transport media (Cary-Blair media) at ambient temperature up to 4 days. 
  Unacceptable: Nonsterile or leaking container, multiple specimens (more than one in 24 hours), dry specimens, delayed transport without use of appropriate preservative, submission of diapers, use of buffered glycerol saline transport media, or refrigerated/frozen stool 
  Specimen Stability: Unpreserved at ambient conditions, 2 hours; preserved at ambient conditions, 4 days 
  Reference Interval: Negative for Vibrio species 
  Reportable Disease: Isolation of toxigenic (i.e., cholera toxin-producing) Vibrio cholerae O1 or O139 from stool is reportable 
  Comments: All suspected Vibrio cholerae are submitted to the NPHL for serogrouping to determine if toxigenic serogroups O1 or O139 are present. The etiological agent of a case of cholera should be reported as V. cholerae O1 or V. cholerae O139. All confirmed toxigenic V. cholerae are submitted to the CDC for serotyping. 
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.