Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Viral Culture, General 
  Synonym: Culture, General Virus Isolation 
  Method: Cell Culture 
  Availability: Daily for specimens received by 1300 
  Specimen: Cerebral spinal fluid, eye, stool, throat, tissue, lesions, tracheal aspirate, BAL, urine, and blood 
  Collection Device: Viral transport swab; commercial viral transport medium for nasopharyngeal wash and aspirate; sterile container for urine, CSF, stool, and BAL; and sodium heparin tube for blood 
  Volume: CSF, 0.5 mL minimum; nasopharyngeal wash and aspirate, 2.0 mL minimum; urine and BAL, 2.0 mL minimum; and blood, 5.0 mL adult / 2.0 mL children. 
  Storage/Transport: Refrigerated 
  Unacceptable: Samples in non-viral transport media, bacteria culture swabs, dry swabs, wood shaft or calcium alginate swabs, samples in EIA and ProbeTec transport media 
  Specimen Stability: Refrigerated, 72 hours 
  Reference Interval: No CPE detected at 14 days 
  Reportable Disease: A positive CSF (any virus), Influenza A virus, Influenza B virus, RSV, or varicella zoster virus result is reportable 
  Revised: 7/1/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.