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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Chlamydia trachomatis Culture 
  Synonym: CT Culture  
  Method: Cell Culture/Immunofluorescence 
  Availability: Performed daily, reported 2-5 days 
  Specimen: Cervical, eye, rectal, urethral swab, or peritoneal fluid.
Also acceptable for newborns: Nasopharyngeal aspirate, swab or washing  
  Collection Device: Immediately place swab, fluid, or washing in 3 mL universal transport medium such as M4, M4RT, M5, M6, UniTranz-RT, or UTM (available at NPHL client services)

Specimen Collection:
1. Aseptically remove sterile swab from package
2. Collect specimen by vigorously swabbing site involved
3. Aseptically remove cap from vial
4. Insert swab into medium and break swab at the scored line
5. Replace cap to vial. Close tightly
6. Fill out vial label with patient's name, date of birth, and source of specimen
7. Refrigerate during transport
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: Swab or 3ml fluid  
  Storage/Transport: Prior to and during shipment: Frozen on Dry Ice
Shipping instructions, including specimen-handling requirements during transport:
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Frozen specimen should be shipped on dry ice. Transport by NPHL ground courier (NPHL brown shipper should not be used, not valid for Dry Ice transport). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: 
  Unacceptable: Urine. Specimens in any transport media other than indicated. Calcium alginate, dry, or wood swabs 
  Specimen Stability: Ambient: 1 h; Refrigerated: 48 h; (Frozen at -20°C: Unacceptable); Frozen at -70°C: 1 month 
  Reference Interval: Culture negative for Chlamydia trachomatis. 
  Reportable Disease: Positive for Chlamydia trachomatis should be reported within 7 days to local or state health department.  
  Comments: Specimen source required.
Not recommended for routine detection of Chlamydia trachomatis (CT) from endocervical or urethral, rectal, vaginal or urine specimens (see PCR test). Use to detect CT in medicolegal settings and to assess suspected treatment failure. May be considered for anatomic locations for which amplified testing has not been validated.  
  Revised: 12/18/2018 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.