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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Dengue Fever Virus Antibodies, IgG & IgM 
  Synonym: Dengue fever virus serology 
  Method: Enzyme-Linked Immunosorbent Assay 
  Availability: Daily; Results in 4 to 14 days 
  Specimen: Blood or CSF 
  Collection Device: Clot (Red) or SST (Gold) tube (blood) Sterile, leak-proof container (CSF) 
  Volume: 1 - 4 mL blood (0.5 - 2 mL of serum), > 1 mL CSF 
  Storage/Transport: Separate serum from the cells ASAP. Transport and store serum refrigerated; if transport > 48 hours, freeze 
  Unacceptable: Severly lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles 
  Specimen Stability: After separation of serum from cells: ambient, < 8 hours; refrigerated, up to 48 hours; frozen, 1 year 
  Reference Interval: Refer to CDC report 
  Reportable Disease: A positive serological result is reportable 
  Comments: A CDC Data and Specimen Handling (DASH) form must be completed for submission of specimens to the CDC.

Appropriately labeled acute and convalescent specimens, if available, should be sent together. The ideal times of collecting serum for serology are 3 - 10 days after onset of symptoms for acute and 2 - 3 weeks after acute sample for convalescent.

Laboratory criteria for a confirmatory diagnosis are:
i) isolation of dengue fever virus from or demonstration of specific arboviral antigen or genomic sequences in tissue, blood, cerebrospinal fluid, or other body fluid by PCR test, immunofluorescence or immunochemistry, ii) seroconversion from negative for dengue virus-specific serum IgM antibody in an acute phase (≤ 5 days after symptom onset) specimen to positive for dengue-specific serum IgM antibodies in a convalescent-phase specimen collected ≥ 5 days after symptom onset, iii) demonstration of a ≥ 4-fold rise in reciprocal IgG antibody titer in paired acute and convalescent sera, or iv) virus-specific IgM antibodies demonstrated in CSF. 
  Revised: 2/2/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.