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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

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  Test: Dengue Virus Antibodies, IgM
Order in NUlirt or complete NPHL Test Order Form  
  Synonym: Arbovirus, Dengue virus serology  
  Method: Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)  
  Availability: Test performed Monday-Friday ; Turn-around time 10 days  
  Specimen: Serum and cerebrospinal fluid (CSF)  
  Collection Device: SST(Gold) tube,
CSF collection tube

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. "Mark specimen plainly as "acute or convalescent."

Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
  Volume: Serum:1 mL;   Minimum:0.5 mL (additional testing for Zika and chikungunya requires separate containers); 
CSF 1.0 mL  
  Storage/Transport: Prior to and during shipment: Refrigerate 2-8°C. Centrifuge and aliquot serum into sterile leak-proof tube, ASAP.  Place both CSF and serum at 2-8°C.
Shipping instructions, including specimen handling requirements during transport:
Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
Ship as Category B, refrigerated in insulated box on frozen gel-packs.  Place packing list or other paperwork on top of Styrofoam and lid. Mark out rigid box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens  
  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles  
  Specimen Stability: 2-8°C for 5d,  frozen 1yr  
  Reference Interval: Negative - No significant level of detectable dengue virus IgM antibody.
Equivocal - Questionable presence of antibodies.  Repeat testing in 10-14 days may be helpful.
Positive – IgM antibody to dengue fever virus detected, which may indicate a current or past infection.  
  Reportable Disease: A positive result is reportable as Arbovirus infection under Title 173, report within 7 days. Refer to Nebraska DHHS Title 173, Communicable Diseases  
  Comments: Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.

May be used to diagnose dengue during acute phase of disease (>5 days after symptom onset). Testing should also be considered for other arthropod-borne viruses with similar symptomology based on clinical presentation and travel history.

Documentation must accompany the specimen to include the following information:  symptoms and date of onset, pregnancy status, travel and vaccine history. 
Serological tests can cross react with other Flavivirus, such as West Nile virus.
Recent vaccinations for Yellow fever virus, Japanese encephalitis virus, and Tick-borne encephalitis virus can cause cross reactive test results. Natural infections with St. Louis encephalitis virus and West Nile virus can also cause cross reactive results. 

Test performed at the CDC.  
  Revised: 3/25/2021 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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