Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at nphl@unmc.edu or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

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  Test: Hepatitis B Surface Antigen 
  Synonym: Australian Antigen; HAA; HBsAg; Hepatitis Associated Antigen 
  Method: Chemiluminescent Immunoassay 
  Availability: Daily; results in 24 hours 
  Specimen: Blood 
  Collection Device: 6.0 mL SST (Gold) tube or 5.0 mL EDTA (Lavender) tube 
  Volume: 2.0 mL serum or plasma (Min: 1.0 mL) 
  Storage/Transport: Refrigerate; if transport to lab is not same day as collected, aliquot the serum or plasma into a clean tube and label as "serum" or "plasma." 
  Unacceptable:  
  Specimen Stability: After separation from cells: Ambient, 8 hours; refrigerated, 14 days (if aliquoted to clean tube); frozen, 1 year (avoid multiple freeze/thaw cycles) 
  Reference Interval: Nonreactive 
  Reportable Disease:  
  Comments: Reactive samples will be confirmed by neutralization. Confirmatory testing is recommended on all reactive (positive) samples collected for prenatal screening. If patient fits this criteria, please call Regional Pathology Services to request confirmatory testing. 
  Revised: 7/12/2011 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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