Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Hepatitis B Surface Antigen  
  Synonym: Australian Antigen; HAA; HBsAg; Hepatitis Associated Antigen 
  Method: Chemiluminescent Immunoassay  
  Availability: Daily; results in 24 hours  
  Specimen: Blood  
  Collection Device: 6.0 mL SST (Gold) tube or 5.0 mL EDTA (Lavender) tube  
  Volume: 2.0 mL serum or plasma (Min: 1.0 mL)  
  Storage/Transport: Refrigerate; if transport to lab is not same day as collected, aliquot the serum or plasma into a clean tube and label as "serum" or "plasma."  
  Specimen Stability: After separation from cells: Ambient, 8 hours; refrigerated, 14 days (if aliquoted to clean tube); frozen, 1 year (avoid multiple freeze/thaw cycles)  
  Reference Interval: Nonreactive  
  Reportable Disease:  
  Comments: Reactive samples will be confirmed by neutralization. Confirmatory testing is recommended on all reactive (positive) samples collected for prenatal screening. If patient fits this criteria, please call Regional Pathology Services to request confirmatory testing. 
  Revised: 7/12/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.