Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at nphl@unmc.edu or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

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  Test: Antibiotic Resistance Confirmation Test 
  Synonym: Broth dilution, Disk diffusion, Gradient Diffusion 
  Method: Molecular and/or Culture-based Methods 
  Availability: Test performed Monday - Friday 
  Specimen: Actively growing isolated organism in pure culture  
  Collection Device: Organism in pure culture on agar slant or plate
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: Not Applicable 
  Storage/Transport: Prior to and during shipment: Ambient 15-25°C
Shipping instructions, including specimen-handling requirements during transport:
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: http://nphl.org 
  Unacceptable: Nonviable organism, mixed culture, leaking container 
  Specimen Stability: Stable at Ambient 15-25°C  
  Reference Interval: Susceptible, Intermediate or Resistant
MIC in ug/ml  
  Reportable Disease: VRSA, and VISA are reportable immediately  
  Comments: Sites submitting specimens for testing must be approved by the Epidemiology Program at DHHS Regulation and Licensure. Please call NPHL for clarification at 402-559-2440 or 866-290-1406. Indicate source of organism, identification test results, susceptibility testing results, and any other pertinent information. Isolates may be requested for confirmation of VRSA, VISA and other highly resistant organisms by the Nebraska State Epidemiology Program.
See Carbapenemase Molecular Detection for KPC and CRE testing  
  Revised: 12/10/2018 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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