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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: ShigaToxin EIA (E. coli)  
  Synonym: Shigatoxin testing, E. coli 0157H7, Shiga-like toxin EIA 
  Method: Enzyme-linked immunosorbent assay  
  Availability: Test performed daily; Reported as soon as positive detected or Final: Negative at 2 days  
  Specimen: Stool  
  Collection Device: Stool container or enteric transport maintenance media e.g., Cary-Blair or Enteric Plus.Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: 10 mL stool in enteric transport media. Fill to line on maintenance media container.  
  Storage/Transport: Prior to and during shipment: Preserve in enteric transport maintenance media at ambient or 2-8°C.

Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address:  
  Unacceptable: Non-sterile or leaking container, multiple specimens ( more than 1 in a 24 hour period), dry specimen, delayed transport  
  Specimen Stability: Ambient or 2-8°C up to 4 days.  
  Reference Interval: Negative  
  Reportable Disease: Positive results are reported xx to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send approval to or call (402) 559-9444.

The shigatoxin EIA test can be used to determine “proof-of-cure” for individuals previously positive for shigatoxin positive E.coli in the stool. Write “proof-of-cure” in comments when ordering via NUlirt or the NPHL Test Order Form.

All shigatoxin EIA-positive stools should be submitted to the NPHL for culture. 
  Revised: 7/22/2019 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.