||Chikungunya Virus RNA Detection
||Arbovirus, Chikungunya virus RT-PCR
||RT-Polymerase Chain Reaction (PCR)
||Test performed Monday-Friday; Turn-around time 1-3 weeks
||Serum and cerebrospinal fluid (CSF)
| Collection Device:
||Clot (Red) or SST(Gold) tube
CSF collection tube
Specimen Labeling: Test subject to CLIA regulations and require 2 patient identifiers on specimen container and requisition forms.
||Serum: 1 mL (0.25 mL minimum). Additional testing for dengue and Zika requires separate containers.
CSF: 1.0 mL
||Centrifuge and aliquot serum into sterile leak-proof tube, ASAP. Place both CSF and serum at 4°C.
Shipping instructions, including specimen handling requirements during transport: Sender should contact the NPHL by email or phone before shipping. Ship specimen Monday -Thursday overnight to avoid weekend deliveries. Refrigerated, specimen should be shipped on cold packs. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address: http://nphl.org/. Do not send directly to CDC.
||Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles
| Specimen Stability:
||2-8 °C for 72 hours, if > 3 days, freeze
| Reference Interval:
||NEGATIVE - No significant level of detectable chikungunya virus RNA
POSITIVE – Chikungunya virus RNA detected
| Reportable Disease:
||A positive result is reportable as Arbovirus infection under Title 173, report within 7 days
||Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.
Supplemental Forms Required - NPHL Special Microbiology Requisition must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history.
During the first 7 days of illness, viral RNA can often be identified in serum and RT-PCR is the preferred test. Therefore, to definitively rule out the diagnosis, convalescent-phase samples should be obtained from patient whose acute-phase samples test negative. Refer to Chikungunya Virus Antibody IgM test for specimen collection after 7 days.