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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at nphl@unmc.edu or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

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  Test: Dengue Virus RNA Detection 
  Synonym: Arbovirus, dengue virus RT-PCR 
  Method: RT-Polymerase Chain Reaction (PCR) 
  Availability: Test performed Monday-Friday; Turn-around time 1-3 weeks 
  Specimen: Serum and cerebrospinal fluid (CSF) 
  Collection Device: Clot (Red) or SST(Gold) tube
CSF collection tube

Specimen Labeling: Test subject to CLIA regulations and require 2 patient identifiers on specimen container and requisition forms. 
  Volume: Serum: 1 mL (0.25 mL minimum). Additional testing for dengue and Zika requires separate containers.
CSF: 1.0 mL 
  Storage/Transport: Centrifuge and aliquot serum into sterile leak-proof tube, ASAP. Place both CSF and serum at 4°C.

Shipping instructions, including specimen handling requirements during transport: Sender should contact the NPHL by email or phone before shipping. Ship specimen Monday -Thursday overnight to avoid weekend deliveries. Refrigerated, specimen should be shipped on cold packs. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address: http://nphl.org/. Do not send directly to CDC. 
  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles 
  Specimen Stability: 2-8 °C for 72 hours, if > 3 days, freeze 
  Reference Interval: NEGATIVE - No significant level of detectable dengue virus RNA
POSITIVE – Dengue virus RNA detected 
  Reportable Disease: A positive result is reportable as Arbovirus infection under Title 173, report within 7 days 
  Comments: Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

Supplemental Forms Required - NPHL Special Microbiology Requisition must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history.


During the first 7 days of these illnesses, viral RNA can often be identified in serum, and RT-PCR is the preferred test for all chikungunya, dengue and Zika viruses. Refer to Dengue Fever Virus Antibody IgM test for specimens collected after 7 days. 
  Revised: 2/5/2016 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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