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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Zika Virus Antibody IgM  
  Synonym: Arbovirus, Arbo-Serology, Zika virus serology 
  Method: ELISA 
  Availability: Test performed Monday-Friday; Turn-around time 1-3 weeks 
  Specimen: Serum and cerebrospinal fluid (CSF) 
  Collection Device: Clot (Red) or SST(Gold) tube
CSF collection tube

Specimen Labeling: Test subject to CLIA regulations and require 2 patient identifiers on specimen container and requisition forms. 
  Volume: Serum: 1 mL (minimum:0.5 mL). Additional testing for Chikungunya and dengue requires separate containers.
CSF: 1.0 mL 
  Storage/Transport: Centrifuge and aliquot serum into sterile leak-proof tube, ASAP. Place both CSF and serum at 4°C.

Shipping instructions, including specimen handling requirements during transport: Sender should contact the NPHL by email or phone before shipping. Ship specimen Monday -Thursday overnight to avoid weekend deliveries. Refrigerated, specimen should be shipped on cold packs. Package and ship as Category B, Biological Substance, UN3373. See instructions and shipping address: Do not send directly to CDC. 
  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-inactivated serum; multiple freeze thaw cycles 
  Specimen Stability: 2-8 °C for 72 hours, if > 3 days, freeze 
  Reference Interval: Negative – No significant level of detectable Zika IgM antibody.
Equivocal – Borderline levels of IgM antibody to Zika virus. (Repeat testing in 10-14 days recommended).
Positive – IgM antibody to Zika virus detected, reflexed for confirmation testing. 
  Reportable Disease: A positive result is reportable as Arbovirus infection under Title 173, report within 7 days 
  Comments: Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

Supplemental Forms Required - NPHL Special Microbiology Requisition must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history.

Substantial serological cross-reactivity exists among the flaviviruses (dengue virus, St. Louis encephalitis virus and West Nile virus) and current IgM antibody assays cannot reliable distinguish among them. Therefore an IgM positive result in a Zika IgM ELISA test will be reflexed for confirmation testing using plaque-reduction neutralization testing which will result in a delay in the reporting of final results.

Molecular detection of Zika virus RNA can be performed on specimens collected at <7days of symptom onset. Refer to the Zika Virus RNA Detection test directory for additional information.  
  Revised: 2/5/2016 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.