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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at nphl@unmc.edu or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

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  Test: Molecular Detection of Carbapenemase 
  Synonym: CARBAR, KPC Detection, CRE 
  Method: PCR; Cepheid Xpert Carba-R 
  Availability: NPHL Special Microbiology Requisition and Special Microbiology Supplemental Form Required. Test performed Monday-Friday. 
  Specimen: Carbapenem Resistant Organism Guidelines:
  • Submission of all isolates of Enterobacteriaceae that are non-susceptible (intermediate or resistant) to any of the carbapenems
  • Exceptions are Enterobacter cloacae and E.aerogenes: only submit isolates that are non-susceptible to Carbapenems other than ertapenem
  • Submission of all non-mucoid isolates of Pseudomonas aeruginosa that are non-susceptible to carbapenems other than ertapenem from non-cystic fibrosis patients
 
  Collection Device: Isolate area on media slant or plate, sealed with parafilm or tape.

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms 
  Volume: One agar or slant 
  Storage/Transport: Prior to shipment: Ambient air

Shipping instructions, including specimen handling requirements during transport: Package and ship ambient as Category B, Biological Substance, UN3373 using shipping material provided by NPHL. See instructions and shipping address: http://nphl.org . If courier not available, contact Client Services (866) 290-1406 to arrange pickup. 
  Unacceptable: Organisms that do not meet the requirements outlined on the supplemental requisition or submitted without the supplemental requisition 
  Specimen Stability: Stable at ambient temperatures 
  Reference Interval: N/A 
  Reportable Disease: All carbapenemase-resistant Enterobacteriaceae will be reported immediately to local or state health department. 
  Comments: Testing results are to be used for epidemiological and infection control purposes only. The confirmation of a carbapenemase-resistant Enterobacteriaceae need to be reported immediately to the in-house infection control official. 
  Revised: 1/24/2017 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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