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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

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  Test: Escherichia coli(Hemorrhagic) Culture, Stool
Order in NUlirt or complete NPHL Test Order Form  
  Synonym: Culture, Hemorrhagic E.coli; Culture, E.coli 0157: H7; Culture, Shigatoxin Positive E. coli  
  Method: Standard reference procedure for confirmation of identification of an isolate for Hemorrhagic E.coli 0157:H7 or Shigatoxin positive E.coli non-o157 by culture or isolation of a Shigatoxin positive E.coli from a Shigatoxin and EIA stool.  
  Availability: Daily  
  Specimen: Shigatoxin positive stool in enteric transport media preferred, or pure culture of E.coli sorbitol-negative isolate on MacConkey or MacConkey with sorbitol plate for identification.  Positive Shiga EIA stool in enteric transport media, fill to line.  
  Collection Device: Sterile container or stool maintenance media 
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
  Volume: 10 mL stool in enteric transport media.  
  Storage/Transport: Prior to and during shipment:  Unpreserved stool at ambient 15-25°C; if transport delayed > 2h, preserve in enteric transport media (Cary-Blair media) at ambient 15-25°C up to 4d. 
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.
Ship as Category B.  Place packing list or other paperwork on top of Styrofoam and lid. Mark out rigid box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address: http://nphl.org/
NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.  
  Unacceptable: Non-sterile or leaking container, multiple specimens ( more than 1 in a 24 hour period), dry specimen, delayed transport to lab without use of appropriate preservative or red transport media indicator has turned yellow. Diapers are not acceptable.  Isolates submitted on blood agar plates may lose toxin expression and are therefore not preferred transport.  
  Specimen Stability: Unpreserved stool stable at Ambient 15-25°C < 2h, preserve in maintenance media at ambient 15-25°C up to 4 days.  
  Reference Interval: Reported as soon as positive detected or Final: Negative at 2 days.  
  Reportable Disease: Report toxicogenic E.coli to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases  
  Comments: Pre-approval needed from state or local health department.  Send approval to NPHL@unmc.edu or call (402) 559-9444.
Note: To test for Shiga toxin from E.coli in stool, see order for Stool Culture or Shiga Toxin EIA. 

NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.  
  Revised: 3/25/2021 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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