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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Cyclospora & Cystoisospora ( Isospora) Modified Acid Fast Stain 
  Synonym: Parasitology Stain by Modified Acid-Fast, Oocyst 
  Method: Concentration procedure with modified acid-fast stain or autofluorescence  
  Availability: Monday, Wednesday, Friday if received by 0800 day of testing; results within 24 hours
(Frequency may be increased during outbreak testing) 
  Specimen: Fresh stool or if transport > 2 h; Proto-fix vial. Add bloody or watery portions of stool if present. Stools fixed in 10% formalin are also acceptable. 
  Collection Device: Use Proto-fix vial for preservation and add stool to fill line marked on Proto-fix vial. Seal and mix well.
Specimen Labeling:Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms 
  Volume: Walnut size stool specimen or 10 ml of stool in preservative. 
  Storage/Transport: Prior to shipment & during Transport: Ambient 15-25°C
Shipping instructions, including specimen-handling requirements during transport:
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: 
  Unacceptable: Dry specimen, leaking container, specimen contaminated with oil, barium or urine, rectal swab, multiple specimens (> 1 in a 24 hour period), and delayed transport. 
  Specimen Stability: Preserved specimen stable for up to 9 months at ambient conditions 15-25°C 
  Reference Interval: No ova or parasites seen 
  Reportable Disease: Cryptosporidium & Cyclospora will be reported within 7 d to local or state health department. 
  Comments: Proto-fix vials available from Client Services.
If the patient has had foreign travel or continued symptoms, One Proto-fix vial can be used for both modified AFB and O&P tests.

This test is recommended for the examination of stool for the presence of Cyclospora or Isospora (Cystoisospora) by modified acid-fast stain in outbreak settings only. Recommended testing for Cryptosporidium is by use of the GI Panel. 
  Revised: 5/13/2019 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.