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Testing Results
IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A Special Microbiology Form must accompany the specimens. Some tests require additional forms and patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.


  Test: Blood Culture, Aerobic and Anaerobic 
  Synonym: Blood Culture 
  Method: Continuous monitoring system; standard reference procedures for identification of aerobic and anaerobic microorganisms and yeast 
  Availability: Daily; preliminary report at 1 day and final report at 5 days for negatives.
All positive blood cultures are reported as soon as detected. 
  Specimen: Blood 
  Collection Device: Bactec Plus Aerobic /F bottle and Bactec Lytic/Anaerobic F bottle, draw aseptically per Blood Culture Collection procedure 
  Volume: 3.0 (minimum) - 10.0 mL of blood each in aerobic bottle and anaerobic bottle, optimum is 10 mL each. If less blood is obtained, split the amount between bottles. For Pediatric patients, 1.0 - 3.0 mL of blood in Bactec Pediatric aerobic culture bottle. 
  Storage/Transport: Transport to lab immediately at ambient 
  Unacceptable: Improperly collected or stored culture bottles 
  Specimen Stability: Specimen should be sent to lab as soon as possible 
  Reference Interval: No growth at 5 days 
  Reportable Disease:  
  Comments: Gram stain performed on postives. Organism identification is performed when clinically-relevant organisms are suspected. Antimicrobial susceptibilities are performed on clinically-relevant isolates when appropriate, following methods recommended by CLSI. Call laboratory for list of routine susceptibility panels. State source of blood if other than peripheral (e.g. line draw) on the test request form. No more than 3 blood cultures sets should be drawn within a 24 hour period, unless from multiples lines. Note if patient is suspected of having Brucella, endocarditis, or any other unusual organism. 
  Revised: 7/7/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.