Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.



AFB MDDR Susceptibility Testing

Supplemental Forms Required – Mycobacterium tuberculosis (MTB) Supplemental


MTB DST, TB, Tuberculosis, MTBC


Targeted DNA Sequencing (Pyrosequencing or Sanger sequencing based on submission criteria provided by submitter), Agar Proportion DST, MGIT 960 Pyrazinamide (PZA) also performed for sediments and isolates,


Monday - Thursday


Nucleic Acid Amplification positive (NAA+) sediment; pure culture isolate on solid medium or in broth culture; Mixed cultures known to contain MTBC; DNA

  Collection Device:

Sediment in centrifuge tube or leak-proof container. Isolate on Lowen-Stein Jensen media

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms


0.5 mL (sediment)


Prior to and during shipment: Refrigerated at 2-8 °C. Place each individual specimen in a sealed bag.

Shipping instructions, including specimen-handling requirements during transport: Sender must contact the NPHL by email or phone before shipping. All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal, Fluids that have potential to be Category A must be packaged UN 2814 Infectious Substances, affecting humans (Category A) and shipped via FedEx. Omaha & Lincoln laboratories can ship via NPHL ground courier by calling client services (866) 290-1406 to request exclusive Category A courier.

See instructions and shipping address:


Sediment should not be shipped frozen.

  Specimen Stability:

Sediment should be refrigerate at 2-8°C, keep isolate at room temperature.

  Reference Interval:

Susceptible or Resistant

  Reportable Disease:

MTB susceptibilities will be reported immediately to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.


Pre-approval is required for this test using the Molecular Detection of Drug Resistance Request Form

Samples with low numbers of MTBC may not amplify; Heteroresistance may not be detected; the results of MDDR assay should not be used to rule out the presence of MTBC in a sample. TAT ranges from 1-6 calendar days

Molecular Detection of Drug Resistance Request Form (CDC-002-00220) . Provide the following Specimen Information on the CDC 50.34 Specimen Submission Form.

  Revised: 9/4/2020 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.