Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

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  Test:

AFB MDDR Susceptibility Testing

Supplemental Forms Required – Mycobacterium tuberculosis (MTB) Supplemental

 
  Synonym:

MTB DST, TB, Tuberculosis, MTBC

 
  Method:

Targeted DNA Sequencing (Pyrosequencing or Sanger sequencing based on submission criteria provided by submitter), Agar Proportion DST, MGIT 960 Pyrazinamide (PZA) also performed for sediments and isolates,

 
  Availability:

Monday - Thursday

 
  Specimen:

Nucleic Acid Amplification positive (NAA+) sediment; pure culture isolate on solid medium or in broth culture; Mixed cultures known to contain MTBC; DNA

 
  Collection Device:

Sediment in centrifuge tube or leak-proof container. Isolate on Lowen-Stein Jensen media

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms

 
  Volume:

0.5 mL (sediment)

 
  Storage/Transport:

Prior to and during shipment: Refrigerated at 2-8 °C. Place each individual specimen in a sealed bag.

Shipping instructions, including specimen-handling requirements during transport: Sender must contact the NPHL by email or phone before shipping. All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal, Fluids that have potential to be Category A must be packaged UN 2814 Infectious Substances, affecting humans (Category A) and shipped via FedEx. Omaha & Lincoln laboratories can ship via NPHL ground courier by calling client services (866) 290-1406 to request exclusive Category A courier.

See instructions and shipping address: http://nphl.org/

 
  Unacceptable:

Sediment should not be shipped frozen.

 
  Specimen Stability:

Sediment should be refrigerate at 2-8°C, keep isolate at room temperature.

 
  Reference Interval:

Susceptible or Resistant

 
  Reportable Disease:

MTB susceptibilities will be reported immediately to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.

 
  Comments:

Pre-approval is required for this test using the Molecular Detection of Drug Resistance Request Form

Samples with low numbers of MTBC may not amplify; Heteroresistance may not be detected; the results of MDDR assay should not be used to rule out the presence of MTBC in a sample. TAT ranges from 1-6 calendar days

Molecular Detection of Drug Resistance Request Form (CDC-002-00220) http://www.cdc.gov/tb/topic/laboratory/MDDRsubmissionform.pdf . Provide the following Specimen Information on the CDC 50.34 Specimen Submission Form.

 
  Revised: 9/4/2020 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 


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