Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Fungus Culture  
  Synonym: Mycology Culture   
  Method: Standard reference procedures for fungal culture. DNA probes available to test isolate for Histoplasma, Coccidioides, and Blastomyces.  
  Availability: Test performed Daily. Preliminary report at 2 weeks, final report at 4 weeks.  
  Specimen: Tissue, pus, purulent fluid, respiratory specimens, body fluid or any body site or fluid, skin biopsy.  
  Collection Device: Collect in a sterile container or a Culturette swab.
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
  Volume: 5.0 mL fluid minimum  
  Storage/Transport: Prior to and during shipment:  Transport and store all sterile body sites (fluids, tissues, etc.) at ambient 15-25°C; nonsterile sites (respiratory, GI tract etc.) at 2-8°C.
Shipping instructions, including specimen-handling requirements during transport:
Ship as Category B.  Place packing list or other paperwork on top of Styrofoam and lid. Mark out rigid box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address:
NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.  
  Unacceptable: Non-sterile, insufficient volume or leaking container.  
  Specimen Stability: Sterile body sites (fluids, tissues, etc.) at ambient 15-25°C; 
Nonsterile sites (respiratory, GI tract etc.) at 2-8°C.  
  Reference Interval: No fungi isolated at 4 weeks.  
  Reportable Disease: Refer to Nebraska DHHS Title 173, Communicable Diseases  
  Comments: Pre-approval needed from state or local health department.  Send approval to or call (402) 559-9444.
Indicate suspected organism(s) on the test request form. Additional patient history may be helpful. Include the patient’s occupation, history of travel or residence abroad, and any animal contact. Media is available for direct inoculation. 
If CSF cannot be transported within 8 hours, hold CSF at 35°C until the time of transport. For blood specimens, refer to Culture, Fungal Blood.
Notification of positive results for mold or positive CSF cultures is provided. For skin, hair, nail specimens, see Culture, Fungus-Dermatophyte order.
NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.  
  Revised: 3/25/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.