Test: Antibiotic Resistance Confirmation Test 
  Synonym: Broth dilution, Disk diffusion, Gradient Diffusion 
  Method: Molecular and/or Culture-based Methods 
  Availability: Test performed Monday - Friday 
  Specimen: Actively growing isolated organism in pure culture  
  Collection Device: Organism in pure culture on agar slant or plate
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: Not Applicable 
  Storage/Transport: Prior to and during shipment: Ambient 15-25°C
Shipping instructions, including specimen-handling requirements during transport:
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address: http://nphl.org 
  Unacceptable: Nonviable organism, mixed culture, leaking container 
  Specimen Stability: Stable at Ambient 15-25°C  
  Reference Interval: Susceptible, Intermediate or Resistant
MIC in ug/ml  
  Reportable Disease: VRSA, and VISA are reportable immediately  
  Comments: Sites submitting specimens for testing must be approved by the Epidemiology Program at DHHS Regulation and Licensure. Please call NPHL for clarification at 402-559-2440 or 866-290-1406. Indicate source of organism, identification test results, susceptibility testing results, and any other pertinent information. Isolates may be requested for confirmation of VRSA, VISA and other highly resistant organisms by the Nebraska State Epidemiology Program.
See Carbapenemase Molecular Detection for KPC and CRE testing  
  Revised: 12/10/2018