NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - NUlirt batch list of NPHL Test Request Form (completed in its entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at 402-559-2440 or toll free at 800-334-0459 for further clarification.
BACK | PRINT
| Test: |
Proof of Cure, Shiga toxin-producing Escherichia coli
- Pre-approval needed from state or local health department
- Send documented approval to NPHL@unmc.edu or call 402-559-9444
- NUlirt Order: SHIGA
|
| Synonym: |
Proof of cure, Shigatoxin testing, E. coli O157:H7, Non O157 |
| Method: |
Qualitative Enzyme Immunoassay |
| Availability: |
Daily |
| Specimen: |
|
| Collection Device: |
- Stool specimens should be collected in Cary Blair or Enteric Plus transport media according to manufacturer's instructions
- If fresh stool is received, it should be placed into transport media within 2 hours of collection
- Ratio of stool to Enteric Plus is 2:15 (0.5 mL stool to 3.75 mL Enteric Plus liquid media)
- Isolates should be on a plate or slant
- Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms
|
| Volume: |
10 mL in transport media |
| Storage/Transport: |
- For shipping of isolates, see Category A shipping instructions
- For shipping of stool specimens, see Category B shipping instructions
- Specimen Packaging & Shipping - NPHL
- Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
- NUlirt packing list or appropriate paperwork (completed in its entirety) must accompany specimens
|
| Unacceptable: |
- Leaking specimen
- Mixed isolate
- Stool contaminated with urine
|
| Specimen Stability: |
Stool stable at 2-8 °C up to 4 days |
| Comments: |
Test performed at Nebraska Medicine |
| Revised: |
10/24/2025 |
If you have questions about proper specimen collection, please call
NPHL Client Services at 402-559-2440 or toll free at 800-334-0459.
BACK
