Bacillus anthracis  

 
Forms Required: NPHL Test Request Form (completed in entirety). It must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel, and vaccine history. Testing will not be performed without all required information. Jim demo purpose

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Bacillus anthracis  
  • Contact the NPHL emergency pager (402) 888-5588 for guidance if Bacillus anthracis cannot be ruled out in the laboratory
  • Perform only required LRN protocol to rule out and refer potentially hazardous pathogens according to LRN-B guidlines 
  • NPHLBenchGuide_FINAL20131221.pdf
 
Synonym: anthrax  
Method: CDC Laboratory Response Network approved methods  
Availability:
  • Test performed Monday-Friday
  • Turnaround time 1-7 days
 
Specimen: Isolates, whole blood (EDTA or sodium citrate), serum, plasma, pleural fluid, respiratory specimens (transtracheal aspirates, bronchial lavage), CSF, clinical swabs, environmental samples  
Collection Device:
  • Isolate on media slant or plate.
  • Seal with Parafilm or tape.
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
 
Volume:
  • Requested volume for liquid clinical samples: 1.0 mL
  • Minimum liquid clinical sample: 0.5 mL
 
Storage/Transport:
  • Category A packaging according to DOT and IATA Category A UN2814 guidlines.
  • Category A packages can only be  transported by a Category A certified courier. 
  • https://www.nphl.org/index.cfm/specimen-packaging-shipping/
  • NUlirt packing list or appropiate paperwork (completed in entirety) must accompany specimens
 
Specimen Stability: Stable at ambient air 14o-25o C  
Reportable Disease:  
Comments: Contact NPHL emergency pager before sending specimen (402) 888-5588  
Revised: 6/23/2025 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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