Haemophilus influenzae (NPHLBK) (CDCSO)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - NUlirt batch list of NPHL Test Request Form (completed in its entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at 402-559-2440 or toll free at 800-334-0459 for further clarification.

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Test: Haemophilus influenzae  
Synonym: H. influenza, H. flu, Flu, Haemophilus  
Method:
  • Banking
  • PCR for serotyping
 
Availability:
  • Test performed Monday-Friday
  • Turnaround time 2 - 5 days for serotyping
 
Specimen: Isolates from sterile sites only and CSF  
Collection Device:
  • Isolate on media slant or plate
  • Cerebral Spinal fluid
  • Test subject to CLIA regulations and requires 2 patient identifiers on container and requistion forms
 
Volume: 500 µL of CSF  
Storage/Transport:
  • See Category B shipping instructions
  • Specimen Packaging & Shipping - NPHL
  • Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
  • NUlirt packing list or appropriate paperwork (completed in its entirety) must accompany specimens
 
Unacceptable:
  • Incorrect storage
  • Non-sterile site isolate
 
Specimen Stability:
  • Isolate stable at ambient air 15°-25° C
  • CSF stable at -20° C
 
Comments:
  • Serotyping performed at the Minnesota Department of Health, Public Health Laboratory, St. Paul, MN
  • Submit all specimens through the NPHL, NOT directly to Minnesota
 
Revised: 10/22/2025 
If you have questions about proper specimen collection, please call NPHL Client Services at 402-559-2440 or toll free at 800-334-0459.

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