NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - NUlirt batch list of NPHL Test Request Form (completed in its entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at 402-559-2440 or toll free at 800-334-0459 for further clarification.
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| Test: |
Molecular Detection of Mpox
- No pre-approval needed
- NUlirt order: MPOX
|
| Synonym: |
Monkeypox, Non-variola orthopox, Orthopox, Orthopoxvirus |
| Method: |
Cepheid Xpert Mpox Assay |
| Availability: |
- Monday - Friday
- Turnaround time 1-2 days
|
| Specimen: |
- Swabs of lesion in viral transport media (VTM/UTM)
- Test subject to CLIA regulations and requires 2 patient identifiers on container and requisition forms
|
| Collection Device: |
Viral transport media (VTM/UTM)
- Collection Procedure:
- Identify two suspected mpox lesions, preferably from two different locations on the body
- Label two tubes containing 3 mL of viral transport medium (VTM/UTM) with two patient identifiers, swabbing location, date and time of collection, and collectors initials
- Using a sterile swab, apply firm pressure to the lesion and swipe the swab back and forth at least 2-3 times before rotating the swab and repeating using the other side of the swab. If the lesion ruptures while swabbing, ensure to collect some of the lesion fluid on the swab as well
- Remove and place each swab into a separate tube containing 3 mL of viral transport medium (VTM/UTM). Break swab at the indicated break line (if necessary) and cap the specimen collection tube tightly to ensure it does not leak in transport
- Mix by inverting rapidly 5 times
|
| Volume: |
- Requested volume for liquid clinical samples: 1 mL
- Minimum volume for liquid clinical samples: 0.5 mL
|
| Storage/Transport: |
- See Category B shipping instructions
- Specimen Packaging & Shipping - NPHL
- Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
- NUlirt packing list or appropriate paperwork (completed in its entirety) must accompany specimens
|
| Unacceptable: |
- Swabs collected in inappropriate transport media
- Inappropriate specimen type
- Incorrect storage
|
| Specimen Stability: |
- Refrigerated 2-8 °C up to 7 days in transport medium
|
| Revised: |
10/24/2025 |
If you have questions about proper specimen collection, please call
NPHL Client Services at 402-559-2440 or toll free at 800-334-0459.
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