NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - NUlirt batch list of NPHL Test Request Form (completed in its entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at 402-559-2440 or toll free at 800-334-0459 for further clarification.
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| Test: |
SARS-CoV-2, Influenza, and RSV Multiplex Assay RT-PCR
- No pre-approval needed
- NUlirt Order: NCOV4
|
| Synonym: |
COVID, Novel coronavirus, RSV, Respiratory Syncytial virus, Influenza A, Flu A, Influenza B, Flu B |
| Method: |
Qualitative nucleic acid amplified real time RT-PCR assay |
| Availability: |
- Test performed Monday - Friday
- Turnaround time 24-48 hours
|
| Specimen: |
- Nasopharyngeal swab
- Nasal swab
|
| Collection Device: |
- Viral Transport Media (VTM, UTM, VCM or M4)
- Specimens should be collected only on mini-tipped swabs with a synthetic tip (such as polyester or Dacron®) with aluminum or plastic shafts
- Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inactivate some viruses and inhibit PCR testing
- Test subject to CLIA regulations and requires 2 patient identifiers on specimen container and requisition forms
|
| Volume: |
1-3 mL viral transport media |
| Storage/Transport: |
- See Category B shipping instructions
- Specimen Packaging & Shipping - NPHL
- Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
- NUlirt packing list or appropriate paperwork (completed in its entirety) must accompany specimens
|
| Unacceptable: |
- Incorrect storage
- Inappropriate specimen type
- Insufficient specimen volume
- Swabs submitted with calcium alginate or cotton tips with wooden shafts
|
| Specimen Stability: |
- Stable refrigerated 2-8° C up to 7 days
- Frozen -20° C up to 30 days
|
| Revised: |
10/24/2025 |
If you have questions about proper specimen collection, please call
NPHL Client Services at 402-559-2440 or toll free at 800-334-0459.
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