AFB Mycobacterium tuberculosis Whole Genome Sequencing Test (TBWGS)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Mycobacterium tuberculosis complex: Drug Resistance Detection & Species Identification by Whole Genome Sequencing  
Synonym: MTB WGS, TB, Mycobacterium tuberculosis, MTBC, Drug Resistance  
Method:
  • See California Department of Public Health (CDPH) Microbial Diseases Lab (MDL) Test Directory for Methodology, reflex testing, specimen types, collection and stability, labeling, volume, rejection criteria, interferences and limitations, and turnaround times
  • Mycobacterium tuberculosis complex: Whole Genome Sequencing
 
Availability: Monday-Thursday  
Storage/Transport:
  • For shipping of isolates, see Category A instructions
  • Specimen Packaging & Shipping - NPHL
  • Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
 
Reportable Disease: See Nebraska Reportable Diseases Title 173 Regulations   
Comments:
  • Submit all specimens for MTB DST through NPHL, NOT directly to CDPH
  • Ship to NPHL on Monday through Thursday to guarantee arrival to CDPH by Friday
  • Test performed at Microbial Diseases Laboratory, California Department of Public Health, 850 Marina Bay Parkway, Richmond, CA 94804
 
Revised: 10/22/2025 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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