Organophosphorous Nerve Agent Metabolites, Serum (OPNAS)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Synonym: Organophosphorous Nerve Agent Metabolites, Serum (OPNAS)
  • For emergency Response/Chemical Terrorism only
  • Contact NPHL emergency pager before sending specimen (402) 888-5588
 
Method: High-Performance Liquid Chromatography Tandem Mass Spectrometry (HPLC MS/MS)  
Availability:
  • Emergency response only
  • Reported within 24 hours of receipt
 
Specimen: Serum  
Collection Device: Collect serum as soon as possible after an exposure incident  
Volume: Optimum: 1.5 mL  
Storage/Transport:
  • Freeze immediately
  • Transport on dry ice 
 
Unacceptable:
  • Low volume (<1.5 mL)
  • Inappropriate storage temperature
  • Damaged collection containers
 
Specimen Stability: Up to 3 years at -70 ºC  
Reference Interval: Organophosphorous Nerve Agents are not normally found in humans  
Comments: Contact NPHL emergency pager before sending specimen (402) 888-5588  
Revised: 10/24/2025 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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