Norovirus RNA Direct Detection (NVOBD) (NPHLBK)  

 
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: Norovirus RNA Direct Detection
  • Pre-approval needed from state or local health department
  • Send documented approval to NPHL@unmc.edu or call (402) 559-9444
  • NUlirt orders: NVOBD and NPHLBK
   
Synonym: Norwalk virus, Human Calicivirus PCR, Norovirus PCR  
Method:
  • Cepheid Gene Xpert Norovirus Assay
  • Whole Genome Sequencing and/or subtyping performed for epidemiology purposes only
 
Availability:
  • Test performed Monday – Friday
  • Turnaround time 24-48 hours
 
Specimen: Stool   
Collection Device:
  • Sterile Container
  • Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms
 
Volume: Minimum 1 mL  
Storage/Transport:
  • See Category B shipping instructions
  • Specimen Packaging & Shipping - NPHL
  • Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material
  • NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens
 
Unacceptable:
  • Stool stored at room temperature >8 hours
  • Stool contaminated with urine
 
Specimen Stability:
  • Stable at ambient air 15°-25° C up to 8 hours
  • Refrigerated 2°-8° C up to 3 days
  • Frozen -20° C up to 1 month
 
Comments: Testing performed on suspected outbreak specimens after state approval for epidemiology purposes only  
Revised: 10/24/2025 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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