Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.

IMPORTANT! Sites requesting testing must have approval from the appropriate state or local health department before submitting specimens. Approval must be sent to NPHL by email at or by calling (402) 559-9444.

A NPHL Test Request Form must accompany the specimens. Certain tests require additional supplemental forms with patient history. This will be listed below when applicable. When requested, testing will not be performed without all required information.

NUlirt Electronic Order Entry Instructions for Collecting and Handling Laboratory Specimens from Nebraska Patients with Suspected COVID-19 5/27/2020

Other NPHL Forms

2021 CDC Specific Submission Form: 50.34 Form

Molecular Detection of Carbapenemase Supplemental Form

Mycobacterium tuberculosis (MTB) Supplemental Form

Vaccine Preventable Disease (VPD) Form

Please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406 for further clarification.

Test Directory                    Specimen Collection Guidelines               Organism List of Category A & B


A  .  B  .  C  .  D  .  E  .  F  .  G  .  H  .  .  J  . K  . L  . M  .  N  . O  .  P  .  Q  .  R  .  S  .  .  U  .  V  .  .  X  .  . Z

NPHL Forms

AFB / Mycobacterium Identification
AFB Culture, Blood
AFB Culture w/ Smear
AFB MDDR Susceptibility Testing
AFB Mycobacterium tuberculosis Amplified Direct Detection
AFB Mycobacterium tuberculosis Direct Susceptibility Test
Arbovirus Antibodies, IgG and IgM, Serum or CSF
Arbovirus RNA Detection 

Bacillus anthracis
Banking (including Confirmation) of Cryptosporidium or Cyclospora Positive Stools 
Blood Culture, Aerobic and Anaerobic
Bordetella pertussis Culture
Bordetella pertussis DNA Detection
Borrelia burgdorferi Antibodies, Total
Botulism Laboratory Confirmation
Brucella species
Burkholderia mallei
Burkholderia pseudomallei

Carbapenemase Molecular Detection
Chlamydia trachomatis / Neisseria. gonorrhoeae DNA

Chikungunya Virus Antibody, IgM
Chikungunya Virus RNA Detection
Copper, Serum (CUBLD)
Culture, Corynebacterium diphtheria
Coronavirus Novel COVID-19 Real-Time RT-PCR Panel for Detection
Coronavirus-19 and Influenza Multiplex Assay RT-PCR
Cryposporidium, Cyclospora & Cystoisospora (formerly Isospora), Modified Acid-Fast Stain
Culture, Aerobic with Gram Stain
Culture, Anaerobic and Aerobic with Gram Stain
Cyanide, Whole Blood (CTCYN)

Dengue Virus Antibodies, IgG & IgM
Dengue Virus RNA Detection

Escherichia coli (Hemorrhagic) Culture, Stool
Francisella tularensis

Fungus Culture
Fungus Culture, Blood
Fungus Culture, Dermatophyte
Fungus Culture, General

Gastrointestinal Pathogen Panel
Gonorrhea Culture (GCSCR)

Hepatitis B Core Antibody, Total (IgG, IgM)
Hepatitis B Surface Antibody
Hepatitis B Surface Antigen
Hepatitis C Antibody
Herpes Simplex Virus Culture
Herpes Simplex Virus Panel, Types 1 And 2
Herpes Simplex Virus (HSV) 1 & 2, DNA Detection and Genotype, Blood or Other Specimens
HIV 1,2 Antibody Confirmation
HIV-1 RNA Quantitation, Ultrasensitive
HIV Antigen/Antibody Screen

Influenza Virus RNA Detection
Lead, Filter Paper Blood Spot (LEADBS)
Lead, Capillary Whole Blood (LEDCAP)
Lead, Venous Whole Blood (LEDVEN)

Legionella Culture
Legionella pneumophila Antigen, Urine

Malaria Exam, Blood
Measles Virus Antibody, IgG
Measles Virus Antibody, IgM
Measles Virus PCR Detection
Metals, Blood (Lead, Cadmium and Mercury, Whole Blood) (BM)
Metals, Urine (Lead, Cadmium and Mercury) (TET, UM)
Mercury, Urine (HG-U)
Mumps Virus Antibody, IgG
Mumps Virus Antibody, IgM
Measles Virus Antibody, IgG and IgM 
Mumps Virus Culture
Mumps Virus PCR Detection
Mumps Virus RNA Detection
Mycobacterium tuberculosis Complex Genotyping
Mycobacterium tuberculosis DNA Amplified Direct Detection

Neisseria meningitides Serotyping
Norovirus RNA Direct Detection
Organophosphorous Nerve Agent Metabolites, Serum (OPNAS)
Organophosphosphorous Nerve Agent Metabolites, Urine (OPNA)

Ova & Parasite, Stool, Complete, Foreign Travel
Ova and Parasite, Stool for Cyclospora & Isospora (Cystoisospora) Stain

Reportable Diseases, Isolate Confirmation
Respiratory Pathogens Panel

Respiratory Pathogen Panel by Multiplex PCR
Respiratory Syncytial Virus Direct Antigen Detection
Rocky Mountain Spotted Fever Antibodies, IgG & IgM
Rotavirus Antigen
Rubella Virus Antibody, IgG
Rubella Virus Antibody, IgM
Rubella (German or Three Day Measles) PCR Detection
Selenium, Serum (SELB)

ShigaToxin EIA (E. coli) Proof-of-Cure, SHIGA
Shigatoxin EIA (E. coli) 
Shigella Serotyping
Stool Culture
Syphilis IgG

Syphilis, Treponemal Antibody Fluorescent, IgG 

TB Interferon Antigen Response
Tetramine, Urine (TETRA)
Treponemal Antibody (FTA) Absorption, IgG

Vibrio Culture
Volotile Organic Compounds, Serum (CTVOC)

West Nile Virus Antibody, IgG & IgM, CSF
West Nile Virus Antibody, IgG & IgM, Serum
West Nile Virus Antibody IgG & IgM, Serum or CSF

Yersinia culture
Yersinia pestis
Zika Virus Antibody IgM
Zinc, Serum (ZINCS)

If you have questions about other tests that are not listed here, please call
NPHL Client Services at 402-559-2440 or toll free at 1-866-290-1406.

Last Updated April 1, 2023.