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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Herpes Simplex Virus Panel, Types 1 And 2
Order in NUlirt or complete NPHL Test Order Form 
  Synonym: HAA; HBsAg; Hepatitis Associated Antigen
  Method: Multiplex Flow Immunoassay  
  Availability: Monday - Friday, Results in 24h  
  Specimen: Blood  
  Collection Device: One 6mL SST (Gold) tube or One 6mL clot (Red) tube.
(EDTA or Heparinized Plasma acceptable but not preferred).
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
  Volume: 1mL serum or plasma (0.5 mL minimum)  

Prior to and during shipment:  Refrigerate 2-8°C. If sample is not transported to lab the same day as collected, aliquot the serum or plasma. To aliquot: Centrifuge the sample and transfer into a clean tube and label as "serum" or "plasma."
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address:

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

  Unacceptable: Body fluids, heat-inactivated, hemolyzed, lipemic or contaminated specimens.  
  Specimen Stability: After separation from cells: Ambient: <8 hours; Refrigerated: 7d; For longer storage, freeze at -20º Celsius. (avoid more than 2 freeze/thaw cycles)  
  Reference Interval: Negative
Equivocal:  Specimen is in the equivocal range. Retesting on a new specimen is recommended.
  Reportable Disease: Immediately report positive Hepatitis B results to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.   
  Comments: Pre-approval needed from state or local health department.  Send approval to or call (402) 559-9444.
Test is intended for the qualitative detection and differentiation of IgG antibodies to HSV-1 and HSV-2.

NUlirt packing list or NPHL Test Order Form (completed in entirety) must accompany the specimen.  
  Revised: 6/11/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.