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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: West Nile Virus Antibody IgG & IgM, Serum or CSF
Order in NUlirt or complete NPHL Test Order Form with Supplemental Form  
  Synonym: WNV antibody, WNV serology, Arbovirus  
  Availability: Note: Contact the NPHL for guidance if arbovirus is suspected. 

For more information, please review the current clinical guidelines for West Nile Virus testing at for serum and for CSF 

Submit all specimens through the state health departments for testing at the CDC, the CDC does not accept routine shipments on weekends or holidays. Therefore, ship to NPHL on Monday – Thursday, to guarantee arrival by Friday.
  Specimen: Serum and CSF  
  Collection Device: See above links.
Specimen Labeling: Test subject to CLIA regulations and requires 2 patient identifiers and specimen source on container.  
  Volume: See above link.  

Centrifuge and aliquot serum into sterile leak proof tube, within 2h of collection. 
Prior to and during shipment: Refrigerate 2-8°C; If transport of sample to the lab is not the same day as collected, frozen specimens are recommended.   

Shipping instructions, including specimen-handling requirements during transport:  Seal tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated or dry ice to keep specimen frozen. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  

See instructions and shipping address:

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

  Unacceptable: Specimen with repeated freeze/thaw cycles.  Hemolysis can cause non-specific binding in serological tests and can have an effect on laboratory results.  
  Specimen Stability: See above link.  
  Reference Interval: IgG: Negative 
IgM: Negative  
  Reportable Disease: Report IgM positive West Nile Virus results within 7d to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases.  
  Comments: Pre-approval needed from state or local health department.  Send documented approval to or call (402) 559-9444.
The Nebraska State Epidemiologist does not recommend diagnosing for acute West Nile IgG antibody only. May be useful for determining past infections/exposure. 
NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens  
  Revised: 7/15/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.