Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Culture, Herpes Simplex  
  Synonym: Herpes Culture; Herpes Simplex 1 and 2 Culture; HSV Culture 
  Method: Cell Culture  
  Availability: Daily; results in 1-7 days  
  Specimen: Vesicle fluid and cells from lesion or CSF; for other sources order Viral Culture, General  
  Collection Device: Starswab Multitrans Collection and Transport System or equivalent

Specimen Collection:
1. Aseptically remove sterile swab from package.
2. Collect specimen by vigorously swabbing site involved.
3. Aseptically remove cap from vial.
4. Insert swab into medium and break swab at the scored line.
5. Replace cap on vial. Close tightly.
6. Fill out vial label with patient's name, date of birth, and source of specimen.
7. Refrigerate during transport.  
  Storage/Transport: Refrigerate  
  Unacceptable: Samples in non-viral transport media, bacterial culture swabs, dry swabs, wood shaft or calcium alginate swabs, samples in EIA and Probetec transport media  
  Specimen Stability: Stable at refrigerated  
  Reference Interval: No CPE detected  
  Reportable Disease:  
  Revised: 2/13/2012 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.