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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Rubella Virus Antibody, IgG  
  Synonym: Rubella antibody titer, German measles antibody 
  Method: Semi quantitative immunoassay  
  Availability: Test performed daily, Reported within 24 hours  
  Specimen: Whole blood  
  Collection Device: SST (Gold) tube, Clot (Red) tube or EDTA (Lavender) tube.

Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: One 6.0 mL SST (Gold) tube or One 6.0 mL Clot (Red) tube or One 5.0 mL EDTA (Lavender) tube. (Min: 0.5 mL)  
  Storage/Transport: Prior to and during shipment: Refrigerate 4-8°C

Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimen should be shipped with frozen gel-packs. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address:  
  Unacceptable: Contaminated, heat-inactivated, or grossly hemolyzed specimens.  
  Specimen Stability: After separation from cells: Ambient: must not be stored ambient; Refrigerated: 7 days; Frozen: 1 year (avoid more than 5 freeze/thaw cycles)  
  Reference Interval: Not detected  
  Reportable Disease: When paired serum testing is done, a four-fold or greater change in titer between the acute and convalescent serum is reported xx to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send approval to or call (402) 559-9444.

Not recommended as a stand-alone test. Rubella Antibodies, IgG and IgM is preferred.
Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Label specimens plainly as acute or convalescent.

Testing immediately post-exposure is of no value without a later convalescent specimen.
The magnitude of the measured result is not indicative of the amount of antibody present. 
  Revised: 7/22/2019 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.