Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Treponemal Antibody Absorption, IgG  
  Synonym: Fluorescent Treponemal Antibody(FTA), FTA-ABS 
  Method: Indirect Fluorescent Antibody  
  Availability: Test performed on Wednesday by 0800; Results same day.  
  Specimen: serum  
  Collection Device: One 6.0mL SST (Gold) tube
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: 2.0 mL serum; 0.5 mL minimum.  
  Storage/Transport: Prior to and during shipment: Centrifuge the sample and transfer the serum into a clean tube. Refrigerate at 4-8°C

Shipping instructions, including specimen-handling requirements during transport:
All screw-top tubes must be sealed with parafilm or tape. Vacutainer tubes do not require parafilm or tape to seal.
Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Refrigerated specimen should be shipped with frozen gel-packs. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup.
See instructions and shipping address:  
  Unacceptable: Whole blood, improper specimen labeling  
  Specimen Stability: After separation from cells: Ambient: <8 hours. Refrigerated: 48 hours; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
  Reference Interval: Nonreactive  
  Reportable Disease: Positive results are reported to the local or state health department, refer to Nebraska DHHS Title 173, Communicable Diseases 
  Comments: Pre-approval needed from state or local health department. Send approval to or call (402) 559-9444.

Please provide RPR result. If no RPR result available, RPR will be performed prior to FTA. 
  Revised: 7/11/2019 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.