Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Mumps Virus Antibody, IgM  
  Synonym: Mumps Virus Serology 
  Method: Enzyme-Linked Immunosorbent Assay  
  Availability: Monday, Wednesday, Friday; results 1 to 6 days  
  Specimen: Blood  
  Collection Device: One 6.0 mL Clot (Red) or one 6.0 mL SST (Gold) tube  
  Volume: 1 - 4 mL blood (0.5 - 2.0 mL serum)  
  Storage/Transport: Separate serum from the cells ASAP. Transport and store refrigerated, and if transport > 48 hours, aliquot and freeze.  
  Unacceptable: Severely lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles  
  Specimen Stability: After separation from cells: ambient, < 8 hours; refrigerated, 48 hours; frozen, 1 year  
  Reference Interval: ≤ 0.79 IV: NEGATIVE - No significant level of detectable IgM antibody to Mumps virus
0.80 - 1.20 IV: EQUIVOCAL - Borderline levels of IgM antibody to Mumps virus. Repeat testing in 10-14 days recommended.
≥ 1.21 IV: POSITIVE - Presence of IgM antibody to Mumps virus detected which may indicate a current or recent infection.  
  Reportable Disease: A positive result is reportable 
  Comments: While the presence of IgM antibodies suggests current or recent infection, low levels of IgM antibodies may occasionally persist for > 12 months post infection or immunization. 
  Revised: 7/6/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.