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IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.
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Test: |
Mumps Virus Antibody, IgM |
Synonym: |
Mumps Virus Serology |
Method: |
Enzyme-Linked Immunosorbent Assay |
Availability: |
Monday, Wednesday, Friday; results 1 to 6 days |
Specimen: |
Blood |
Collection Device: |
One 6.0 mL Clot (Red) or one 6.0 mL SST (Gold) tube |
Volume: |
1 - 4 mL blood (0.5 - 2.0 mL serum) |
Storage/Transport: |
Separate serum from the cells ASAP. Transport and store refrigerated, and if transport > 48 hours, aliquot and freeze. |
Unacceptable: |
Severely lipemic, icteric or hemolyzed samples; heat-activated serum; multiple freeze thaw cycles |
Specimen Stability: |
After separation from cells: ambient, < 8 hours; refrigerated, 48 hours; frozen, 1 year |
Reference Interval: |
≤ 0.79 IV: NEGATIVE - No significant level of detectable IgM antibody to Mumps virus 0.80 - 1.20 IV: EQUIVOCAL - Borderline levels of IgM antibody to Mumps virus. Repeat testing in 10-14 days recommended. ≥ 1.21 IV: POSITIVE - Presence of IgM antibody to Mumps virus detected which may indicate a current or recent infection. |
Reportable Disease: |
A positive result is reportable |
Comments: |
While the presence of IgM antibodies suggests current or recent infection, low levels of IgM antibodies may occasionally persist for > 12 months post infection or immunization. |
Revised: |
7/6/2011 |
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.
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