Dedicated to protecting the health and safety
of Nebraskans through diagnostic laboratory
science, technology, and education.
Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Respiratory Syncytial Virus Direct Antigen Detection  
  Synonym: RSV Direct 
  Method: Enzymatic Immunoassay  
  Availability: Daily; routine results within 4 hours and stat results within 1 hour of specimen receipt in the Microbiology Laboratory  
  Specimen: Nasopharyngeal wash, aspirate, or swab  
  Collection Device: Place specimen in viral transport media  
  Volume: 3.0 mL wash (Min: 2.0 mL)  
  Storage/Transport: Refrigerate; must be received by laboratory within 72 hours  
  Unacceptable: BAL,sputum, smears, bronchial washings  
  Specimen Stability: Refrigerated, < 72 hours  
  Reference Interval: Negative  
  Reportable Disease:  
  Comments: A negative screen does not rule out RSV. 
  Revised: 7/11/2011 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.