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IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.
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| Test: |
HIV Antigen/Antibody Panel |
| Synonym: |
HIV ELISA screen, HIV screen |
| Method: |
Multiplex flow immunoassay |
| Availability: |
Daily, results in 24 hours |
| Specimen: |
Blood |
| Collection Device: |
6 ml SST (Gold) tube, 6ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube |
| Volume: |
Minimum 0.5 ml serum or plasma |
| Storage/Transport: |
Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube. |
| Unacceptable: |
Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours |
| Specimen Stability: |
After separation from cells: ambient if < 24 hours; refrigerated if > 24 hours up to 14 days; frozen if > 14 days. |
| Reference Interval: |
Nonreactive |
| Reportable Disease: |
Reactive is reportable |
| Comments: |
Reflexed to HIV 1,2 Antibody confirmation testing if antigen/antibody screen is reactive.
Detection of HIV-1 p 24 antigen in the absence of detectable HIV antibody may indicate an acute infection. Confirmation testing is performed using the quantitative HIV RNA assay.
All HIV-2 antibody reactive will be confirmed by the CDC. |
| Revised: |
2/28/2017 |
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If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.
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