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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

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  Test: HIV 1,2 Antibody Confirmation EIA  
  Synonym: HIV confirmation, HIV Monospot 
  Method: Rapid enzyme immunoassay  
  Availability: Monday - Friday, within 72 hours of positive HIV Antigen/Antibody screen  
  Specimen: Blood  
  Collection Device: 6 ml SST (Gold) tube, 6 ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube  
  Volume: Minimum 0.5 ml serum or plasma  
  Storage/Transport: Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube.  
  Unacceptable: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours  
  Specimen Stability: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours  
  Reference Interval: Nonreactive  
  Reportable Disease: Reactive is reportable 
  Comments: Indeterminate results are reflex tested by the HIV RNA assay.

All HIV 2 reactive results will be confirmed by the CDC. 
  Revised: 2/28/2017 

 

If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.

 

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