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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Vibrio Culture
Order in NUlirt or complete NPHL Test Order Form with Supplemental Form  
  Synonym: Cholera culture, Vibrionaceae, Vibriosis  
  Availability: Note: Contact the NPHL for guidance if Vibrio cholerae is suspected. 
For more information, please review the current clinical guidelines for Vibrio testing at:   
  Specimen: Stool  
  Collection Device: Preserve 5 mL stool in enteric transport media (Cary-Blair) immediately.
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  

Prior to and during shipment:  Stable at  Refrigerate 2-8°C
Shipping instructions, including specimen-handling requirements during transport:  Seal screw-top tubes with Parafilm™ or tape and place in biohazard bag with absorbent material.

Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container filled with frozen gel-packs to keep specimen refrigerated. Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.  
See instructions and shipping address:

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

  Unacceptable: Nonsterile or leaking container, multiple specimens in 24h period, dry specimens, diapers, or delayed transport to lab in inappropriate preservative.  
  Specimen Stability: Stable at Refrigerated 2-8°C for 72h  
  Reference Interval: Culture negative for Vibrio spp  
  Reportable Disease: Report all positive Vibrio cholerae immediately and all non-V cholerae ssp results from stool with 7d to local and state health department and isolates should be forwarded to the NPHL, refer to Nebraska DHHS Title 173, Communicable Diseases  
  Comments: Pre-approval needed from state or local health department.  Send documented approval to or call (402) 559-9444.
Use to diagnose Vibrio-associated diarrhea in patients with appropriate exposure history or risk factors.

Indicate suspected organisms and available patient history, especially any recent travels, on the test request form. Maintenance media is available from Client Services. 

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens. Test performed at the CDC.  Submit all specimens to the CDC through the NPHL, NOT directly to the CDC.

For more information, please review the current clinical guidelines for Cholera testing at:     
  Revised: 6/21/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.