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of Nebraskans through diagnostic laboratory
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Testing Results
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.


  Test: Gonorrhea Culture
Order in NUlirt or complete NPHL Test Order Form  
  Synonym: GC Screen, Gonococcal Culture, GC Screen  
  Method: Standard reference procedure for Neisseria gonorrhoeae culture and identification.  
  Availability: Test performed Daily.  Preliminary report as soon as found positive, final for negative results at 3 days.  
  Specimen: Any site or fluid, urethral swab, endocervical swab, rectal swab or throat swab.  
  Collection Device: JEMBEC System of Modified Thayer Martin agar sealed in a plastic bag with a CO2 generating tablet, follow package insert directions.   Note specific site on specimen and test request form. 
Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms  
  Volume: Swab  

Prior to and during shipment: Ambient 15-25°C; if any delay in transport, incubate at 37°C.
Shipping instructions, including specimen-handling requirements during transport:  Seal with Parafilm™ or tape and place in biohazard bag with absorbent material.
Place specimen inside leak proof secondary container (biohazard bag and if transporting by air, use Tyvek™ Envelope) with adsorbent material. The secondary container must be packed inside an approved Category B shipping container.  Position NUlirt packing list or NPHL Test Order form between secondary container and box lid. Seal with tape.  Label outer box as UN3373 Category B, Biological Substance. Transport by NPHL ground courier. If courier not available, ship by FedEx, UPS, or USPS (1st Class, Priority or Express Mail). Follow appropriate DOT/IATA Regulation Guidelines.  For assistance, call NPHL State Training Coordinator at (402) 559-3590.
See instructions and shipping address:

NUlirt packing list or appropriate paperwork (completed in entirety) must accompany specimens.

  Unacceptable: Delayed transport of the plates in non-CO2 environment. Refrigerated or frozen specimens  
  Specimen Stability: Stable at Incubator Temperature 35-37°C  
  Reference Interval: Negative for Neisseria gonorrhoeae  
  Reportable Disease: Report positive Neisseria gonorrhoeae within 7d to local or state health department. Refer to Nebraska DHHS Title 173, Communicable Diseases  
  Comments: Specimens should be collected prior to the initiation of antimicrobial therapy. Transport immediately at room temperature. If there is any delay in transport, incubate at 37C.  Culture for N. gonorrhoeae is the method of choice in cases of treatment failure and sexual abuse and for non-genital sources. Media and probes are available from client services.

Antimicrobial susceptibility testing performed on all non-genital specimens and genital specimens upon request.    
  Revised: 6/9/2021 


If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (866) 290-1406.